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. 2008 Feb 8;9(3):260–266. doi: 10.1038/embor.2008.8

Figure 4.

Figure 4

Pont co-purifies with the Brahma complex. (A) Purification scheme of Brm-C. (B) Western analyses of Mono Q fractions in which 50 kDa Pont and Rept purify away from the 170 kDa Pont and Brm peak. (C) Western analyses of peak fractions from glycerol gradient sedimentation of Mono Q fraction 28. (D) 4–15% SDS–polyacrylamide gel electrophoresis (silver staining, lane 1) and western analyses (lanes 2–7) of the Brm–Pont peak (glycerol gradient fraction 14). Note that the antibody used in lane 2 (Pont67) does not crossreact with Mor (J. Antao and R. Kingston, personal communication). (E) Western analyses of proteins precipitated from 0.42 M BioRex-70 fraction by antisera to Pont (IP, Pont53; western, Pont67), Osa, Pb, Mor, Pc or rabbit IgG. Input, 2.5 μl (10 μg); IP, 10 μl (1/10). Brm-C, Brahma complex; IP, immunoprecipitation; Pont, Pontin; Rept, Reptin.