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. 2007 Sep 12;407(Pt 1):113–120. doi: 10.1042/BJ20070651

Figure 1. Effect of PIF alone or in combination with amino acids on protein synthesis in murine myotubes after 4 h of incubation.

Figure 1

The amino acids (2 mmol/l) were added 2 h prior to PIF (4.2 nmol/l). The concentration of the amino acids in DMEM was: 0.8 mmol/l for Leu, Ile and Val; 0.4 mmol/l for Phe; 0.34 mmol/l for Arg; and 0.2 mmol/l for Met. The results are means±S.E.M. of three separate determinations. *P<0.05, **P<0.01 or ***P<0.001 compared with control. †††P<0.001 compared with PIF alone.