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. 2007 Sep 12;407(Pt 1):113–120. doi: 10.1042/BJ20070651

Figure 2. Effect of PIF alone or in combination with BCAAs on phosphorylated and total eIF2α.

Figure 2

(A) Western blot analysis of phospho-eIF2α (p EIF2α) in relation to total eIF2α in cytosolic extracts of murine myotubes after incubation with PIF (4.2 nmol/l) for various periods of time, either alone or in the presence of leucine (2 mmol/l). (B) Effect of the BCAA (2 mmol/l) alone or together with PIF on the phosphorylation of eIF2α after 4 h incubation determined by Western blotting. The amino acids were added to the myotubes 2 h prior to PIF. The densitometric analysis represents the means±S.E.M. for three separate Western blots, and is presented as the ratio of the phosphorylated to total eIF2α (ph/tot). *P<0.05 and ***P<0.001 compared with control; †P<0.05 and †††P<0.001 compared with PIF alone.