Figure 3.

WRN nucleolar localization in multiple cell lines. Cells were subjected to double immunolabeling with anti-WRN and anti-nucleolar protein B23, as described in the legend to Fig. 1. In each row, Left shows the anti-WRN signal, Center shows the anti-B23 (anti-nucleolar signal), and Right shows the DAPI (nuclear) signal. (a–c) WI38 75.1, simian virus 40-transformed human fetal lung fibroblast cell line. (d–f) HeLa, human cervical carcinoma cell line. (g–i) PC-3, prostatic adenocarcinoma. (j–l) GCT, fibrous histiocytoma. (m and n) AG07877, lymphoblastoid cell line. (×500.)