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. 2007 Sep 12;407(Pt 1):49–59. doi: 10.1042/BJ20070705

Figure 2. hCTR2 is localized in intracellular vesicles.

Figure 2

(A) Localization of hCTR2 was assessed by direct confocal laser-scanning microscopy in HEK-293T, U2OS and HeLa cells after transient transfection with hCTR2–eGFP. (B) HeLa cells were transiently co-transfected with both hCTR2–vsvG and hCTR2–eGFP constructs. hCTR2–vsvG was immunolabelled with rabbit anti-vsvG antibodies, and secondary labelling was performed with Alexa Fluor® 568 conjugated-goat anti-rabbit and goat anti-mouse antibodies. hCTR2–eGFP was visualized by direct confocal microscopy. Co-localization is indicated by arrowheads.