Figure 1. Deletion and mutational analysis of the human NHE2 promoter.

Functional analyses were carried out with 5′- (A), 3′- (B) and internal- (C) deletion constructs. Luciferase reporter constructs containing the indicated promoter fragments of the NHE2 promoter along with pSV-βgal were transiently transfected into C2BBe1 cells. At 48 h post-transfection the cells were lysed and reporter gene activity was measured and normalized to β-galactosidase activity as indicated in the Experimental section. The luciferase activity of each deletion mutant is shown as a percentage of the activity of the parental plasmid p−85/+150, which was set at 100%. Values are the means±S.E.M., n≥4. *Significantly different from pGL2-basic.