Table 1. Primers used for PCR amplification of the truncated fragments of the NHE2 proximal promoter region.

The direction of the primers is indicated by F (forward) or R (reverse). Oligonucleotides are named by the first nucleotide corresponding to the NHE2 promoter sequence with respect to the TIS. Restriction enzyme recognition sites are shown in bold.

Direction/position	Sequence
F/−85	5′-TATCTCGAGTGTGCCGGCCAGGTCCC-3′
F/−40	5′-TATCTCGAGCCGCGCCCGCCCCGCCC-3′
F/−20	5′-TATCTCGAGCCCGCGGGCCGCCC-3′
F/−10	5′-TATCTCGAGCGCCCTGGGCAGGGC-3′
F/+4	5′-AGCTCGAGCCTGCCGCTGCGGCTGGAGAGC-3′
F/+11	5′-AGCTCGAGCTGCGGCTGGAGAGCAGC-3′
R/+4	5′-TATAAGCTTCCCTGCCCAGGGCGGCCCGC-3′
R/+11	5′-TATAAGCTTCGGCAGGCCCCTGCCCAG-3′
R/+150	5′-TATAAGCTTCCTCGCTGCGAGCAGGCGCAC-3′
R/+71	5′-TATAAGCTTCTCAGCCGCCCTCCGCCGCCG-3′
R/+41	5′-TATAAGCTTCCATGCCGGTGCGCTGCTCTCCAGC-3′