Table 2. Primers used for GMSA and site-specific mutagenesis of the TIS.

Name, sequence and end-points of the oligonucleotides are indicated. Base substitutions are shown in bold. The nucleotide sequences of the sense strands are given.

Name	Sequence	Position
TIS	5′-GCCGCCCTGGGCAGGGCCTGCCGCTGCGGCTG-3′	−12 to +20
TIS-1	5′-GCCGCCCTGGGCAGG-3′	−12 to +3
TIS-2	5′-GCCTGCCGCTGCGGCTG-3′	+4 to +20
TIS-3	5′-GGCAGGGCCTGCCGC-3′	−3 to +12
TIS-4	5′-CCCTGGGCAGGGCCTGCCGC-3′	−8 to +12
TIS-m1	5′-CCCTGGGTTCGGCCTGCCGC-3′	−10 to +12
TIS-m2	5′-CCCTGGGCAGGGCTTTCCGC-3′	−6 to +18
TIS-m3	5′-CTTTGGGCAGGGCCTGCCGC-3′	−8 to +12
m-TIS #1	5′-GCCGCCCTGGGTTCGGCCTGCCGC-3′	−12 to +12
m-TIS #2	5′-CTGGGCAGGGCTTTCCGCTGCGGC-3′	−6 to +18