Table 3. Dynamic range and affinity of cGMP sensors.
Dynamic ranges and affinities (EC50) of all cGMP sensors described so far and reported in this manuscript are shown. To facilitate comparison, either the YFP/CFP (Y/C) or the CFP/YFP (C/Y) emission ratio changes are listed, whichever is larger for a given sensor, i.e. YFP/CFP is given for constructs that show cGMP-induced FRET increases and CFP/YFP for constructs showing cGMP-induced FRET decreases. n.d., value missing in the reference cited.
| cGMP effect | ||||
|---|---|---|---|---|
| Parent protein | Sensor | FRET change (%) | EC50 (μM) | Reference |
| cGK | cgy-Del1 | 24Y/C | n.d. | [29] |
| cygnet | 40C/Y | 1.9 | [30] | |
| cGES-GKIB | n.d. | 5 | [31] | |
| cGi-500 | 77C/Y | 0.5 | − | |
| cGi-3000 | 72C/Y | 3 | − | |
| cGi-6000 | 58C/Y | 6 | − | |
| PDE5 | cGES-DE5 | 16Y/C | 1.5 | [31]* |
*, Supplement.