Table 1.

Triplex PCR primers for amplifying the target genes in E. coli O157:H7 and Vibrio cholerae O139

Target gene	Target organism	Sequence(5'-3')	PCR product length (bp)	Concentration in PCR mixture
Stx1	E. coli O157	F: GAA TTT ACC TTA GAC TTC TCG AC	250	0.15 μmol/L
		R: TCC TGT TAA CAA ATC CTG TCA C		0.75 μmol/L
Stx2	E. coli O157	F: TAC GAT AGA CTT TTC GAC TCA AC	207	0.1 μmol/L
		R: TCA ATA ATC AGA CGA AGA TGG TC		0.5 μmol/L
UidA	E. coli O157:H7	F: TAA TGA GGA GTC CCT TAT GTT AC	179	0.1 μmol/L
		R: ACT GAT CGT TAA AAC TGC CTG G		0.5 μmol/L
CtxA	Vibrio cholerae	F: ACT CAG ACG GGA TTT GTT AGG C	304	0.15 μmol/L
		R: ATC TAT CTC TGT AGC CCC TAT TAC		0.75 μmol/L
TcpA	Vibrio cholerae	F: TTG ACC CAA GCA CAA TGT AAG AC	241	0.15 μmol/L
		R: CTA CTG TGA ATG GAG CAG TTC C		0.75 μmol/L
LPSgt	Vibrio cholerae	F: ACA TCT GTA GGG ATT GTA TTG AC	340	0.2 μmol/L
	O139	R: ATA ACA ACT GAG ATA TCA AGC GTC		1.0 μmol/L