Table 2.

Oligonucleotide probe sequences for identification of E. coli O157:H7 and Vibrio cholerae O139

Target gene	Sequence(5'-3')	Length (bp)	Tm(°C)a
Stx1	GTA CGT CTT TAC TGA TGA TTG ATA GTG GCA CAG GG	35	73.5
Stx2	AGT TAT TTT GCT GTG GAT ATA CGA GGG CTT GAT GT	35	69.6
uidA	TGG AAT TGA GCA GCG TTG G	19	70.1
ctxA	CAT ACA GTC CTC ATC CAG ATG AAC AAG AAG TTT CTG CTT TAG GTG	45	75.0
tcpA	CAG GAA GTG CCA ACT TAA ACC TAA CTA ATA TCA CGC ATG TTG AGA	45	76.8
LPSgt	TCG ATA AGA AGA GAT AAA GAT CTG AGT TAT CTA AAG ATA TTT G	43	71.2
Negative control	TCT TCG CCA GAG GCC TGC TAG CCT GGT TCA AGA TAC TAC C	40	68.5
Labeling control	Complimentary sequence of the reverse primer	20	70.5

^aTm(°C) was calculated using primer premier 5.0