Table 5.

Cutoff value of the probe

Target gene	Species	Average of negative bacteria and blank control signal intensity	SD	Cutoff valuea
ctxA	Vibrio cholerae O139	1783.12	64.27	1847.39
TcpA		1739.338	68.253	1807.591
LPSgt		1637.29	57.175	1694.465
stx1	Escherichia coli	1691.284	71.22	1762.504
Stx2	O157:H7	1790.385	78.473	1868.858
UidA		1802.1746	69.181	1871.3556
16S rDNA	internal control	1598.5629	24.75	1623.3129

^a cutoff value = average of negative bacteria and blank control signal intensity + 2 SD