Figure 5. Zip4h−/Y mice exhibit perturbations with respect to crossover formation.
Numbers of MLH1 foci per cell were tabulated in MLH1 positive spermatocyte spreads from Zip4h+/Y and Zip4−/Y mice and percent spreads containing the indicated foci were graphed (A). To determine whether DSB repair foci persisted to the stage when crossovers form, cells were co-stained with antibodies against MLH1 (red), RAD51 (green) and SCP3 (blue). Representative bivalents from Zip4h+/Y (B), and Zip4h−/Y (C) spreads are shown.
