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Western blot detection of parotid secretory protein in gland lysates prepared from 8- and 20-week-old NOD.Igμnull mice. Fifty micrograms of tissue lysates was separated by SDS-polyacrylamide gels. The normal 25-kDa BALB/c isoform and the NOD-specific ≈20.5-kDa isoform are indicated by asterisks. Sub, submandibular gland; Par, parotid gland.
