Fig. 1.

Replica plating using low-melt agarose. A mixture of 3% low melt agarose containing complete growth medium and serum is poured on a dish with partially trypsinized colonies and allowed to solidify. As shown in the figure, the agarose is removed from the original dish after marking the dish and agarose for orientation and flipped over onto a second dish to transfer part of the colony from the original dish. Only three rather than 50 colonies are shown for illustrative purposes. The dishes are shown prior to marking the replica dish for orientation. After 1 day, the agarose is removed from the replica dish 2