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Concentrations of the five variables of the optimal medium
| Variables | Concentration |
|---|---|
| Glutamine | 2.5 mM |
| EAA | 3.25 × (1.67 g/L) |
| NEAA | 2.125 × (0.168 g/L) |
| ITS | 1 × (0.22 g/L) |
| Lipids | 0.7 × (0.71 g/L) |
Total concentration of all amino acids in EAA and NEAA are given in g/L. Concentrations of all other components are as given in Table 2
