Fig. 3.

Growth of CHO cells in a suspension cell culture and in 800 μm microcapsules. Cultures were performed in a continuous 1.5 L bioreactor, and cell concentration is given with respect to the volume of the reactor. The initial microcapsule volume was 1/15 of the reactor volume. The inoculum concentration was 5 × 10⁴ cell mL⁻¹_reactor for both cultures. The suspension cell culture was undertaken in batch mode. Microapsules were first cultured for 140 h in batch mode before medium feed was started