Table 1.
Kinetic and stoichiometric parameters of suspension and encapsulated CHO cells cultures
| Value | Units | Suspension cells (batch) | Suspension cells + 1.5% alginate (batch) | 800 μm microcapsules liquid core | 500 μm microcapsules liquid core |
|---|---|---|---|---|---|
| μ | (h−1) | 0.028 | 0.021 | 0.027/0.013 | 0.020/0.006 |
| Culture time | (h) | 160 | 80 | 650 | 690 |
| Xreactor, max | (cell mL−1reactor) | 1.41 × 106 | 8.5 × 105 | 2.2 × 106 | 2.9 × 106 |
| Xcaps, max | (cell mL−1caps) | – | – | 6.6 × 107 | 8.7 × 107 |
| Max cells | (cell caps−1) | – | – | 17000 | 6100 |
| Colonization (ϕ) | (%) | – | – | 17 | 20 |
| qS | (mmol cell−1 h−1) | 6.4 × 10–10 | 3.3 × 10−10 | 7.0 × 10−10 | 4.7 × 10−10 |
| qlactate | (mmol cell−1 h−1) | 1 × 10−9 | 6 × 10−10 | 9.7 × 10−10 | 7 × 10−10 |
| qO2 | (mmol cell−1 h−1) | 3.2 × 10−10 | - | - | 5.9 × 10−10 |
| qNH3 | (mmol cell−1 h−1) | 4.7 × 10−11 | - | 8.0 × 10−11 | - |
| qP | (mg cell−1 h−1) | 1.7 × 10−9 | 2.0 × 10−9 | 5.3 × 10−9 | 4.3 × 10−9 |
| YX/S | (cell mmol−1) | 4.0 × 107 | 5.9 × 107 | 7.9 × 106 | 2.3 × 107 |
| Ylactate/S | (mol mol−1) | 1.64 | 1.72 | 1.41 | 1.45 |
The suspension cells were cultivated in batch mode. The 800 μm microcapsules were incubated for 30 min in PLL and the 500 μm microcapsules for 10 min. The specific growth rate (μ) is given for the two growth phases of the perfusion cultures (first phase corresponds to the batch mode and the second phase begins when the feed is initiated). The biomass yield with respect to glucose (YX/S), corresponds to the yield during the growth phase