Table 2.
Kinetic and stoichiometric parameters for 500 mL spinner-flask cultures of encapsulated CHO cells. The maximum cell number (X) is given with respect to the microcapsule volume (Xcaps, max)
| Value | Units | 800 μm capsules with single PLL membrane | 800 μm capsules with a double PLL membrane | 500 μm capsules with a semi-liquid core |
|---|---|---|---|---|
| μ | (h−1) | 0.019 | 0.021 | linear |
| Culture time | (h) | 516 | 698 | 1050 |
| Xcaps, max | (cell mL−1caps) | 5.5 × 107 | 5.5 × 107 | 1.2 × 107 |
| Max. cells per capsule | (cell caps−1) | 10700 | 14700 | 2600 |
| Occupied vol. (ϕ) | (%) | 12 | 13 | 3 |
| qS | (mmol cell−1 h−1) | 4.0 × 10−10 | 4.8 × 10−10 | 3.0 × 10−10 |
| qlactate | (mmol cell−1 h−1) | 6.9 × 10−11 | 8.2 × 10−10 | 5.3 × 10−10 |
| qNH3 | (mmol cell−1 h−1) | 2.2 × 10−11 | – | – |
| qP | (mg cell−1 h−1) | 3.7 × 10−9 | 4.9 × 10−9 | 3.4 × 10−9 |
| Ylactate/S | (mol mol−1) | 1.70 | 1.75 | 1.73 |