Table 2.
Published papers dealing with genomic and proteomic analysis in mammalian cell culture
| Cell line | Comparison | Culture Condition | Analysis | Source | |
|---|---|---|---|---|---|
| Genomic | Proteomic | ||||
| Hybridoma Cell line | Temperature, sparging, agitation | Bioreactor/suspension | − | + | Passini et al. (1989) |
| CHO-K1 | 10% FCS, insulin, basic fibroblast growth factors | adherent/T-flask/batch | − | + | Lee et al. (1996) |
| NS0/NS0_r (cholesterol independent) | Cholesterol-dependent/-independent | Serum-free/suspension/spinner flask/batch | + | + | Seth et al. (2005) |
| CHO | Exponential growth phase—reference map | Serum-free/suspension/2L bioreactor/fed-batch | − | + | Champion et al. (1999) |
| CHO-K1 | 70–80% confluence—reference map | Adherent/T-flask/batch | − | + | Hayduk et al. (2004) |
| CHO-K1 | 0.5 mM n-butyrate acid, 80 μM zinc sulphate | Adherent/T-flask/batch | − | + | van Dyk et al. 2003) |
| Murine hybridoma cells | Hyperosmotic—390 mOsm/kg | Suspension/T-flask/batch | + | − | Shen et al. (2005) |
| CHO | Hyperosmotic—300, 350, 400, 450 mOsm/kg | Adherent/6-well plates/batch | − | + | Lee et al. (2003) |
| MAK mouse-mouse hybridoma cell line | Nutrient feeding—metabolic shift | Serum-free/suspension/fed-batch to continuous culture | − | + | Seow et al. (2001) |
| CHO | 0–2.5% DMSO | Adherent/T-flask/batch | − | + | Li et al. (2006) |
| CHO-K1 | Temperature shift—37°C to 31°C | Adherent/T-flask/batch | − | + | Kaufmann et al. (1999) |
| CHO | Temperature shift—37°C to 33°C | Adherent/T-flask/batch | + | + | Baik et al. (2006) |
| GS-NS0 | Temperature—37°C, 34°C and 22°C | Suspension/T-flask/batch | + | − | Swiderek et al. (2006) |
| CHO-SEAP | High vs low producer | Adherent/T-flask/batch | − | + | Hayduk et al. (2005) |
| GS-NS0/wild type NS0 | Producer vs non-producer | Suspension/spinner flask/batch | + | − | Khoo et al. (2007) |
| GS-NS0 | High vs low producer | Suspension/T-flask/batch | − | + | Alete et al. (2005) |
| GS-NS0 | High vs low producer | Suspension/T-flask/batch | − | + | Smales et al. (2004) |
| GS-NS0 | Mid-exponential phase—reference map | Serum-free/shaker flask/batch | − | + | Smales et al. (2003) |
| GS-NS0 | High vs low producer | Suspension/T-flask/batch | − | + | Dinnis et al. (2006) |
| Murine hybridoma cells | Zinc as insulin replacement | Serum-free/shaker flask/batch | + | − | Wong et al. (2006c) |
| CHO | Batch and fed-batch culture | Serum-free/suspension/5L bioreactor/batch&fed-batch | + | − | Wong et al. (2006a) |