Table 1.
Nutrient content of chemically defined medium.
| Diet | Units per liter |
|---|---|
| Reference | 1.35 g L-methionine/150 g glucose |
| Sugar restricted | 1.35 g L-methionine/50 g glucose |
| High methionine | 4.05 g L-methionine/150 g glucose |
| Low methionine | 0.45 g L-methionine/150 g glucose |
| Other nutrients (constant for all media) | |
| L-aspartic acida | 2.5 g |
| L-glutamic acida | 5.6 g |
| L-serinea | 2.3 g |
| Glycinea | 1.0 g |
| L-histidine HCl-H2Oa | 1.1 g |
| L-arginine HCla | 3.9 g |
| L-threoninea | 2.1 g |
| L-alaninea | 2.6 g |
| L-prolinea | 2.1 g |
| L-tyrosinea | 1.9 g |
| L-valinea | 3.0 g |
| L-cystinea | 1.0 g |
| L-isoleucinea | 1.9 g |
| L-leucinea | 3.1 g |
| L-phenylalaninea | 2.2 g |
| L-lysine HCla | 6.5 g |
| L-tryptophanb | 17.4 g |
| Vitamin B12b | 18.8 μg |
| Biotinc | 0.015 μg |
| P-aminobenzoic acid (PABA)c | 2 mg |
| Inositolc | 42 mg |
| Niacinc | 10 mg |
| Pantothenic acidc | 6 mg |
| Folic acidc | 6 mg |
| Pyridoxine HClc | 3 mg |
| Riboflavinc | 2.4 mg |
| Thiamin HClc | 1.5 mg |
| Cholineb | 16 mg |
| Vitamin Ad | 1350 IU |
| Vitamin Ed | 16.5 IU |
| Vitamin Dd | 335 IU |
| Vitamin K (MSBC)d | 0.5 mg |
| Zince | 11.284 mg |
| Coppere | 4.675 mg |
| Chromiume | 0.5616 mg |
| Potassiumc | 446.1372 mg |
| Phosphorusc | 245.6724 mg |
| Calciumc | 4.6569 mg |
| Chloridec | 16.0476 mg |
| Ironc | 2.58 mg |
| Magnesiumc | 24.2556 mg |
| Manganesec | 3.185 mg |
| Sodiumc | 5.0826 mg |
| Cholesterolc | 10 mg |
| Lecithinc,f | 0.1 g |
| Ribonucleic acid, gc,f | 1.0 g |
a Based on empirical determination of amino acid content in 15% yeast/sugar medium. Analysis conducted by Nutritional & Environmental Analytical Services Laboratory, Cornell University (Ithaca, NY).
b After Hinton et al. (1951) Medium 4.
c After Villee and Bissell (1948).
d Requirements for Drosophila uncertain. Added at 1/3 concentration recommended in American Institute of Nutrition guidelines for rodent diets (AIN-93M).
e Requirements for Drosophila uncertain. Calculated from theoretical nutrient content in 15 grams Brewers yeast.
f Diet does not contain Iodine, Selenium and other trace minerals present in mammalian diets