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Alignment of Silico 3 sequences indicating the insertion of the predicted Helitron Silico3 in A636 and B73. There is no Helitron insertion in McC, W22, W23, or bz-R. It is interesting that, in addition to an occupied site, B73 also shows a weak Silico3 vacant-site-sized band in Fig. 4. Sequencing of this PCR product confirmed it to be an unoccupied site.
