Figure 2.

A perturbation–expression matrix for a set of lineage-specific TFs. The graph indicates the fold increase or decrease (RNAi relative to untreated) and statistical significance (Z-score) for the effect of each TF RNAi (columns) on each TF expression level (rows). The time point with the largest fold-change was selected. Where the fold change is less than 1.3 fold, a small gray circle is shown. The rows and columns are organized by spatial expression pattern of each TF with the same color scheme as in Figure 1. Muscle and epidermal submatrices are outlined by gray and yellow dashed lines, respectively. Positive yeast one-hybrid results are indicated by gray squares and represent the interaction between the TF protein (column) and 1.5 kb of noncoding DNA sequence 5′ of a particular TF gene (row). All yeast one-hybrid combinations were tested with the exception of those involving the promoter of lin-26, which is a member of an operon. tbx-8 and tbx-9 are chromosomal neighbors transcribed in opposing directions, and consequently share a putative promoter region. Positive results for TBX-8 and TBX-9 have been combined (See Supplementary Figure 2 for complete data set).