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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2007 Sep 6;293(5):G995–1003. doi: 10.1152/ajpgi.00537.2006.

Fig. 3.

Fig. 3

Effects of absolute IRS-1 deficiency on mass of colonic mucosa and muscularis in wild-type (WT) mice and mice that overexpress a human IGF-I transgene driven by a mouse metallothionein-1 promoter (MT-hIGF-I trans-genic mice). Histograms show means ± SE for wet weight, DNA, and protein content per centimeter of colonic mucosa (A) or colonic muscularis (B). Muscularis mass in colon in IRS-1+/+/WT (closed bar), IRS-1+/+/MT-hIGF-I (gray bar), IRS-1−/−/WT (hatched bar), or IRS-1−/−/MT-hIGF-I mice (cross-hatched bar) is shown. Numbers above histograms are mean values in MT-hIGF-I transgenics expressed as a percentage of values in WT mice of same IRS-1 genotype to indicate magnitude of MT-hIGF-I-dependent bowel growth. Results of 2-way ANOVA analyses on absolute values are shown, where *P < 0.001 and not significant (NS) = P > 0.05. ANOVA analyses are described in more detail in supplemental material, available on American Journal of Physiology-Gastrointestinal and Liver Physiology website.