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. Author manuscript; available in PMC: 2008 Nov 1.
Published in final edited form as: Am J Physiol Gastrointest Liver Physiol. 2007 Sep 6;293(5):G995–1003. doi: 10.1152/ajpgi.00537.2006.

Fig. 8.

Fig. 8

Expression and phosphorylation of IGF-IR signaling molecules in cultured intestinal mesenchymal cells. Intestinal myofibroblasts were derived from IRS-1+/+ and IRS-1−/− mice as described in EXPERIMENTAL PROCEDURES Cells were serum starved for 24 h and were treated with IGF-I at different doses. Cell extracts were immunoprecipitated with antibodies against IRS-1, IRS-2, IGF-IR, or Shc and were immunoblotted with indicated antibodies. Immunoblots are representative of at least 4 separate experiments.