Table 1.
Degenerate oligonucleotides for PCR amplification of VL and VH of mAb 54.6
| MVH-R | 5'- GAC HGA TGG GGS TGT YGT GCT AGC TGN RGA GAC DGT GA -3' |
| MVK-R | 5'- GGA TAC AGT TGG TGC AGT CGA CTT ACG TTT KAT TTC CAR CTT -3' |
| VK1-F | 5'- GGG GAT ATC CAC CAT GGA GAC AGA CAC ACT CCT GCT AT -3' |
| VK2-F | 5'- GGG GAT ATC CAC CAT GGA TTT TCA AGT GCA GAT TTT CAG -3' |
| VK3-F | 5'- GGG GAT ATC CAC CAT GGA GWC ACA KWC TCA GGT CTT TRT A -3' |
| VK4-F | 5'- GGG GAT ATC CAC CAT GKC CCC WRC TCA GYT YCT KGT -3' |
| VK5-F | 5'- GGG GAT ATC CAC CAT GAA GTT GCC TGT TAG GCT GTT G -3' |
| VH1-F | 5'- GGG GAT ATC CAC CAT GGR ATG SAG CTG KGT MAT SCT CTT -3' |
| VH2-F | 5'- GGG GAT ATC CAC CAT GRA CTT CGG GYT GAG CTK GGT TTT -3' |
| VH3-F | 5'- GGG GAT ATC CAC CAT GGC TGT CTT GGG GCT GCT CTT CT -3' |
| VH4-F | 5'- GGG GAT ATC CAC CAT GAT RGT GTT RAG TCT TYT GTR CCT G -3' |