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. 2008 Mar 18;6(3):e64. doi: 10.1371/journal.pbio.0060064

Figure 5. Induction of GlmY Expression Antagonizes GlmZ RNA Processing.

Figure 5

E. coli MC4100 ΔglmY (JVS-8030) or ΔglmY ΔglmZ (JVS-8113) strains were transformed with control vector, pBAD, or an arabinose-inducible glmY expression plasmid, pBAD-GlmY (as indicated above the panels). Transformants were grown to early stationary phase and treated with 0.2% l-arabinose to induce glmY expression. RNA was prepared prior to or at the indicated time intervals (in minutes) upon induction, and subjected to northern analysis to detect changes in GlmY, GlmZ, and glmS mRNA expression. Black lollipops indicate the primary GlmY or GlmZ transcripts, whereas open lollipops represent the approximately 150-nt processed species of these sRNAs.