Table 2.

Primers for RT-PCR

Primer name:	Sequence 5′-3′	ORF (bp)
AKHR-for	CAG CCA GCC AGC CAG AAC
AKHR-rev	GGA GCG TTA GTA ACA TGG AAT GAA GTG	1110
Actin-for	CCC GCT GAA CCC GAA GGC TAA CC
Actin-rev	GTA CCA CCG GAC AGG ACA G	585
N-term-T7-for	T7– CAG CCA GAA CAA TGC CCA ACA CAA
N-term-T7-rev	T7– GCC AGT ATG CTG AGC ACC GTA A	267
C-term-T7-for	T7– TAT GGT ACT GGC TCG ACA AGG AGT
C-term-T7-rev	T7– ATG TTA CTA ACG CTC CTA CGC CCT	253
T7-sequence	TAA TAC GAC TCA CTA TAG G	–

ORF: open reading frame spanning of the forward (for) and reverse (rev) primer pair; AKHR: adipokinetic hormone receptor of Anopheles gambiae (Kaufmann and Brown, 2006); N-term and C-term-T7 primer set represent the forward and reverse primer set of the N- and C-terminus of the AKHR and T7 sequence was added for dsRNA synthesis.