Skip to main content
. Author manuscript; available in PMC: 2008 Mar 15.
Published in final edited form as: Cancer Cell. 2002 Feb;1(1):53–62. doi: 10.1016/s1535-6108(01)00002-2

Figure 2.

Figure 2

Expression of TVA and susceptibility of ovarian cells from TVA transgenic mice to RCAS infection

A: Immunohistochemical detection of the TVA receptor with the TVA antibody. Ovaries isolated from β-actin-TVA transgenic mice express the TVA receptor in both stromal cells and the ovarian surface epithelium. In ovaries isolated from keratin 5-TVA transgenic mice, expression of the TVA receptor is restricted to the cells of the ovarian surface epithelium.

B: Detection of RCAS-AP-infected cells by AP color reaction. Ovarian cells from each transgenic line were infected with the RCAS-AP virus in culture. Endogenous AP was heat-inactivated, and the infected cells that produce AP were detected by the AP color reaction. Both stromal and epithelial cells from β-actin-TVA ovaries are susceptible to RCAS-AP infection. In ovaries from keratin 5-TVA mice, only the ovarian surface epithelial cells are susceptible to RCAS-AP infection, while stromal cells are AP-negative.