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. Author manuscript; available in PMC: 2008 Mar 16.
Published in final edited form as: Oncogene. 2007 Apr 2;26(42):6194–6202. doi: 10.1038/sj.onc.1210438

Figure 3.

Figure 3

Confirmation of E2F1 sites in the rat Bcl-2 promoter. (a) HEK293 cells were transfected with Bcl-2-P1 plus constructs expressing transcription factors identified in protein-DNA arrays (Figure 2). pSV-β-Gal was used as internal control. Cell lysates were analyzed for luciferase and β-Gal activities 48 h post-transfection. Luciferase activity was normalized to β-Gal to obtain the relative luciferase activity. E2F-1 binding sites were confirmed using (b) deletion analyses and (c) site-directed mutagenesis. Left, diagram of Bcl-2 promoter-luciferase constructs. Right, Bcl-2 promoter activities in the presence and absence of exogenous (b) E2F1 or (c) β-catenin. Data, mean±s.d., n=3. WT, wild-type. *P<0.05; **P<0.01.