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. Author manuscript; available in PMC: 2008 Mar 16.
Published in final edited form as: Oncogene. 2007 Apr 2;26(42):6194–6202. doi: 10.1038/sj.onc.1210438

Figure 4.

Figure 4

Regulation of Bcl-2 promoter activity by β-catenin. (a) Induction of Bcl-2 promoter activity by forced expression of β-catenin. HEK293 cells were transfected with Bcl-2-P1 promoter-luciferase construct alone, or Bcl-2-P1 plus a construct that overexpressed wild-type β-catenin; pSV-β-Gal was used as internal control. Alternatively, endogenous β-catenin was overexpressed with 30mM LiCl. (b) Immunoblot of whole cell lysates showing increased Bcl-2 and β-catenin following exogenous β-catenin or LiCl treatment. (c) Induction of Bcl-2 promoter–reporter activity by wild-type (Wt) β-catenin and β-catenin mutants D32N, G34E and Δ45, with concomitant changes in β-catenin and Bcl-2 protein expression in whole cell lysates.