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. Author manuscript; available in PMC: 2008 Mar 16.
Published in final edited form as: Oncogene. 2007 Apr 2;26(42):6194–6202. doi: 10.1038/sj.onc.1210438

Figure 5.

Figure 5

Knockdown of c-Myc or E2F1 attenuates β-catenin-dependent induction of Bcl-2 promoter activity and Bcl-2 protein expression. HEK293 cells were transfected with Bcl-2-P1, in the presence and absence of exogenous β-catenin, plus c-Myc siRNA or E2F1 siRNA. Luciferase and β-Gal activities were assayed 48 h post-transfection. Luciferase activity was normalized to β-Gal activity to obtain the Relative Luciferase Activity. Data, mean±s.d., n=3; **P<0.01. Corresponding whole cell lysates were immunoblotted for β-catenin, c-Myc, E2F1, β-actin, Bcl-2 and cleaved caspase 3, as indicated. Arrows, 19-kDa and 17-kDa bands indicative of cleaved (active) caspase 3.