Fig. 5.

Antigenotoxic effects of A. keiskei, O. javanica and B. oleracea in HCT116 human colon cancer cells. Cells were incubated in the absence (−) or presence (+) of 0.1 mM H₂O₂ and DNA damage was quantified via the comet assay. Median tail moment in positive controls treated with H₂O₂ but no plant extract was assigned an arbitrary value of ‘1.0’ in order to facilitate comparison among treatment groups. Wedge symbol: increasing concentration of each extract (0, 1, 5, 10, 20 μg/mL) *P < 0.05 and **P < 0.01 by ANOVA.