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. Author manuscript; available in PMC: 2008 Mar 16.

Published in final edited form as: Biochim Biophys Acta. 2007 May 8;1769(7-8):506–513. doi: 10.1016/j.bbaexp.2007.04.009

Ring specific cam-dependent luciferase activity is stimulated by hrp3 region between -1.7 to -1.1. A) Schematic representation of the 1.7 kb hrp3 promoter region depicting the different hrp3 fragments placed upstream of the cam 5’ end region. In addition, the organization of the hybrid promoter is shown. B) Transient luciferase activity was measured in ring and trophozoite parasites and normalized to CAT activity. The control plasmid (pcamSluc) contains cam promoter and firefly luciferase gene (FFL). pAhrpcamSluc, pA1hrpcamSluc, pA2hrpcamSluc represent plasmids with hrp3 A, A1 and A2 fragments placed upstream of the cam promoter respectively. Bars are the mean of three independent experiments ± SD. C) Schematic representation of the 1.7 kb hrp3 promoter region depicting the different hrp3 fragments placed upstream the cam 5’ end region. In addition, the general organization of the hybrid promoter is shown. D) Transient luciferase activity was measured in ring and trophozoite parasites and normalized by cotransfection with a plasmid carrying the Renilla luciferase gene driven by the cam promoter. The control plasmid (pcamSluc) contains the cam promoter driving firefly luciferase gene (FFL). pBhrpcamSluc, pB1hrpcamSluc, pB2hrpcamSluc represent plasmids with hrp3 B, B1 and B2 fragments placed upstream the cam promoter respectively. Bars are the mean of four independent experiments ± SD.

Ring specific cam-dependent luciferase activity is stimulated by hrp3 region between -1.7 to -1.1. A) Schematic representation of the 1.7 kb hrp3 promoter region depicting the different hrp3 fragments placed upstream of the cam 5’ end region. In addition, the organization of the hybrid promoter is shown. B) Transient luciferase activity was measured in ring and trophozoite parasites and normalized to CAT activity. The control plasmid (pcamSluc) contains cam promoter and firefly luciferase gene (FFL). pAhrpcamSluc, pA1hrpcamSluc, pA2hrpcamSluc represent plasmids with hrp3 A, A1 and A2 fragments placed upstream of the cam promoter respectively. Bars are the mean of three independent experiments ± SD. C) Schematic representation of the 1.7 kb hrp3 promoter region depicting the different hrp3 fragments placed upstream the cam 5’ end region. In addition, the general organization of the hybrid promoter is shown. D) Transient luciferase activity was measured in ring and trophozoite parasites and normalized by cotransfection with a plasmid carrying the Renilla luciferase gene driven by the cam promoter. The control plasmid (pcamSluc) contains the cam promoter driving firefly luciferase gene (FFL). pBhrpcamSluc, pB1hrpcamSluc, pB2hrpcamSluc represent plasmids with hrp3 B, B1 and B2 fragments placed upstream the cam promoter respectively. Bars are the mean of four independent experiments ± SD.