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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Exp Parasitol. 2007 Feb 4;116(3):327–333. doi: 10.1016/j.exppara.2007.01.020

Figure 4. Deletion of the hrp3 promoter switched stage specific expression from ring to trophozoite stage.

Figure 4

(A) Schematic representation of hrp3 promoter truncations (diagonal lines) driving human DHFR fused to GFP (HDGFP, filled box) in plasmid pH1.7HG containing the 1.7-kb full length promoter as well as the plasmids pH1.1HG, pH0.6HG carrying the truncated promoters of 1.1-kb and 0.6-kb respectively. (B) Total RNA from stable cell lines expressing HDGFP was purified from ring (i, iii) and trophozoite (ii, iv) stages, separated on agarose gels stained with ethidium bromide (iii, iv) and analyzed by northern blot (i, ii) using 32P-GFP antisense probe.