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. Author manuscript; available in PMC: 2009 Feb 15.
Published in final edited form as: Exp Cell Res. 2007 Nov 17;314(4):887–895. doi: 10.1016/j.yexcr.2007.10.031

Figure 1.

Figure 1

Hypersensitivity of XP-V cells to psoralen photoadducts. A) Whole cell lysates of XP30RO/c15 (XP-V/pol η) and XP30RO/cDNA3 (XP-V/cDNA) cells were analyzed by Western immunoblotting for pol η expression, using actin as a loading control. B) Cytotoxicity following HMT and UVA treatment. XP-V/pol η(•, ■) and XP-V/cDNA (○, □) were exposed to increasing concentrations of either HMT, and then irradiated with either a single dose of 5 kJ/m2 UVA (•,○), or a split-dose UVA protocol consisting of 5 kJ/m2, followed by rinsing with HBSS to remove unbound HMT and then re-irradiation with 15 kJ/m2 (■,□)). Cells were then replaced in media and incubated for 3 days before survival was assessed. Error bars denote standard deviation.