Figure 1.
Awake mouse two-photon microscopy experimental apparatus. A) A two-photon microscope is used to image through the cranial window of an awake behaving mouse that is able to maneuver on an air-supported free-floating Styrofoam ball that acts as a spherical treadmill. Optical computer mice are used to record mouse locomotion by quantifying treadmill movement. B) Images of the disassembled (i) and assembled (top-view (ii) and side-view (iii)) head-plate used for cranial window imaging and mouse head restraint.