Figure 4.

Imaging neural population activity in sensory cortex of awake behaving mice. Ai) False color time-projection image of the 5 minute long time series (~150 µm deep); neurons were loaded with Calcium Green-AM (green channel) and were negative for the astrocytic marker SR101 (red channel). B) Fluorescence traces for the neuropil and 34 neurons outlined in Aii obtained after the time series was motion corrected. Running speed, air puff stimulus and brain displacements are also shown. Red segments indicate >3σ positive going fluorescence transients with <5% false positive error rate (Figure 6E). C) An expanded view of the fluorescence activity of 4 neurons. D) Correlation coefficients of neuron fluorescence versus running speed. The neurons were numbered throughout this figure in descending correlation coefficient order. E) Running onset triggered average of fluorescence for 7 neurons with varying amounts of running correlation. F) Neuron-Neuron fluorescence activity correlation coefficients.