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. 2008 Jan 21;153(Suppl 1):S137–S153. doi: 10.1038/sj.bjp.0707659

Figure 4.

Figure 4

Potential glycogen synthase kinase (GSK3) Ser/Thr-directed phosphorylation relays in nuclear factor of activated T cells (NFATs). (a) Sequences for Homo sapiens NFATs were aligned by CLUSTAL W (1.83). Accession numbers are NFATc1, NP_006153.2 (825 residues); NFATc2, NP_036472.2 (921 residues); NFATc3, NP_004546.1 (1068 residues) and NFATc4, NP_004545.2 (902 residues). Alignment of the ‘atypical' NFAT, NFAT5, has been omitted. The extended and highly conserved Ser/Thr relays (potentially phosphorylated by GSK3) present in the N-terminal regions of NFATc1, NFATc2, NFATc3 and NFATc4 are in underlined, bold italicized type. In NFATc1, these sites are thought to be phosphorylated by GSK3. Other more limited relays are present in individual NFATs and all contain a Ser-rich domain N-terminal to the GSK3 consensus sequences that is phosphorylated by casein kinase 1. (b) GSK3 relays (underlined, bold italicized type) present in GATA4(1–217). (c) GSK3 relays (underlined, bold italicized type) in mouse myocardin (residues 454–466 and 624–636) as identified by Badorff et al. (2005). Identical sequences are present in H. sapiens myocardin. There are also ‘out-of-phase' relays in these regions (shaded, bold and italicized), which are not as strongly conserved in H. sapiens myocardin.