Figure 4.
IFN-γ responsiveness can be restored to SK-LC-7 by enforced expression of the IFN-γR α chain and to SK-LC-2 and SK-LC-19 by expression of Jak2. The ability of cells to enhance expression of MHC class I proteins is measured after 72 hr of incubation with 1,000 IRU/ml of either HuIFN-γ (Left) or HuIFN-αA/D (Right) (thick lines) or buffer (thin lines). (A) SK-LC-7 cells transfected with expression plasmids encoding either the HuIFN-γR α chain (Upper) or HuIFN-γR β chain (Lower). (B) SK-LC-2 cells transfected with plasmids encoding either Jak1 (Upper) or Jak2 (Lower). (C) SK-LC-19 cells transfected with plasmids encoding Jak1 (Upper) or Jak2 (Lower).