Table 1a.

Quantification of CD3+ T-cell CCR5 expression following chronic CCL5 exposure. This table compliments Figure 1a, and demonstrates a significant reduction in the number of CD3+CCR5+ T-cells and CD3+ CCR5 mean fluorescent intensity (MFI), following incubation with CCL5 at 37°C, indicative of receptor internalization following ligand-receptor interactions.

	CD3+ T-cells (% PBMCs)	Number of CD3+ T-cells	CD3+ CCR5+ (% of CD3+ T-cells)	Number of CD3+ CCR5+ T-cells	CD3+ CCR5+ MFI	p-value*
INPUT (PBMCs)	D1= 54.3	499,560	16.0	79,930	39.9
D1=920,000	D2= 45.3	437,145	23.9	104,478	28.9
D2=965,000	Mean= 49.8	468,353	20.0	92,204	34.4	n/a
4°C, No CCL5	D1= 54.1	497,720	16.4	81,626	41.1
	D2= 45.7	441,005	24.3	107,164	29.1
	Mean= 49.9	469,363	20.4	94,395	35.1	n.s.
4°C, + CCL5	D1= 54.8	504,160	13.8	69,574	29.0
	D2= 45.6	440,040	17.6	77,447	19.8
	Mean= 50.2	472,100	15.7	73,511	24.4	n.s.
37°C, No CCL5	D1= 55.2	507,840	16.3	82,778	39.1
	D2= 46.2	445,830	23.8	106,108	28.8
	Mean= 50.7	476,835	20.1	94,443	34.0	n.s.
37°C, + CCL5	D1= 55.1	506,920	8.71	44,153	20.4
	D2= 46.5	448,725	9.24	41,462	16.7
	Mean= 50.8	477,823	8.98	42,808	18.6	0.02

^*indicates that p-values were ascertained relative to the number of input CD3+CCR5+ T-cells (n=4). Key: D1: Donor 1, D2: Donor 2, n/a: not applicable, n.s.: not significant, PBMCs: peripheral blood mononuclear cells.