Table 1b.

Quantification of CD14+ monocyte CCR5 expression following chronic CCL5 exposure. This table compliments Figure 1b, demonstrating a mild reduction in the number of CD14+CCR5+ monocytes and CD14+ CCR5 mean fluorescent intensity (MFI) following incubation with CCL5 at 37°C. This indicates CCR5 internalization occurs following CCL5-CCR5 interactions in vitro.

	CD14+ monocytes (% PBMCs)	Number of CD14+ monocytes	CD14+ CCR5+ (% of CD14+ monocytes)	Number of CD14+ CCR5+ monocytes	CD14+ CCR5+ MFI	p-value*
INPUT (PBMCs)	D1= 15.5	142,600	6.19	8,827	39.9
D1=920,000	D2= 23.3	224,845	7.05	15,852	28.9
D2=965,000	Mean= 19.4	183,723	6.62	12,340	34.5	n/a
4°C, No CCL5	D1= 15.3	140,760	6.39	8,995	39.1
	D2= 22.6	218,090	6.93	15,114	27.4
	Mean= 19.0	179,425	6.60	12,055	33.3	n.s.
4°C, + CCL5	D1= 15.3	140,760	5.52	7,770	30.7
	D2= 22.8	220,020	5.84	12,849	24.9
	Mean= 19.1	180,390	5.68	10,310	27.8	n.s.
37°C, No CCL5	D1= 15.1	138,920	5.89	8,182	41.8
	D2= 22.5	217,125	7.32	15,894	28.7
	Mean= 18.8	178,023	6.61	12,038	35.3	n.s.
37°C, + CCL5	D1= 14.9	137,080	4.33	5,936	29.1
	D2= 22.1	213,265	5.04	10,749	20.7
	Mean= 18.5	175,173	4.69	8,343	24.9	0.04

^*indicates that p-values were ascertained relative to the number of input CD14+CCR5+ monocytes (n=4). Key: D1: Donor 1, D2: Donor 2, n/a: not applicable, n.s.: not significant, PBMCs: peripheral blood mononuclear cells.