Table 2b.

CCR5 expression on CD14+ monocytes during migration across the IVBBB. This table demonstrates the CCR5 mean fluorescent intensity (MFI) values on CD14+ monocytes from 5 different donors. There was no change in MFI on migrated and non-migrated monocytes in either the basal IVBBB or aIVBBB models relative to the input. This table, coupled with the data in Figure 2a, indicates that CCR5 down-regulation on CD14+ monocytes following CCL5-driven migration is based on a reduced percentage of receptor-positive cells, rather than a reduced receptor surface expression per cell.

BBB MODEL	CONDITION	Donor 1	Donor 2	Donor 3	Donor 4	Donor 5	p-value*
	INPUT	22.7	22.6	37.9	28.8	46.1	n/a
Basal IVBBB	No Chemokine-Non-migrated	23.0	21.4	32.9	28.7	44.0	n.s.
Basal IVBBB	No Chemokine-Migrated	23.6	23.0	36.4	30.6	49.7	n.s.
Basal IVBBB	+ CCL5-Non-migrated	20.3	21.3	37.0	27.3	44.0	n.s.
Basal IVBBB	+ CCL5-Migrated	20.7	21.9	33.2	28.1	45.8	n.s.
aIVBBB	No Chemokine-Non-migrated	23.5	21.6	36.9	27.9	44.3	n.s.
aIVBBB	No Chemokine-Migrated	23.4	21.1	34.8	30.4	42.2	n.s.
aIVBBB	+ CCL5-Non-migrated	22.4	22.0	37.2	28.0	43.8	n.s.
aIVBBB	+ CCL5-Migrated	20.7	20.8	37.4	26.2	45.1	n.s.

^*indicates that p-values were ascertained relative to the input MFI. Key: aIVBBB: cytokine-activated in vitro blood-brain barrier, IVBBB: in vitro blood-brain barrier, n/a: not applicable, n.s.: not significant.