Table 2c.

CCR5 expression on CD3+ T-cells during migration across the IVBBB. This table demonstrates the CCR5 mean fluorescent intensity (MFI) on CD3+ monocytes from 5 different donors. There was a significant reduction in CCR5 MFI on T-cells following CCL5-driven migration in either the basal IVBBB or aIVBBB models relative to the input. There was no change in the MFI on non-migrated cells or cells that migrated without added CCL5 in either model relative to the input. This table in addition to the data in Figure 2a, indicates that CCR5 down-regulation on CD3+ T-cells following CCL5-driven migration is associated with a reduced receptor expression per cell.

BBB MODEL	CONDITION	Donor 1	Donor 2	Donor 3	Donor 4	Donor 5	p-value*
	INPUT	25.8	24.4	38.0	28.2	27.9	n/a
Basal IVBBB	No Chemokine-Non-migrated	25.9	24.8	37.9	27.4	22.2	n.s.
Basal IVBBB	No Chemokine-Migrated	23.1	26.6	41.1	27.8	27.5	n.s.
Basal IVBBB	+ CCL5-Non-migrated	24.7	22.4	36.6	31.2	24.6	n.s.
Basal IVBBB	+ CCL5-Migrated	16.4	15.6	27.4	21.9	19.9	0.03
aIVBBB	No Chemokine-Non-migrated	24.4	22.4	39.3	27.2	27.1	n.s.
aIVBBB	No Chemokine-Migrated	20.8	17.0	43.3	26.4	29.8	n.s.
aIVBBB	+ CCL5-Non-migrated	23.8	22.3	38.5	29.8	31.1	n.s.
aIVBBB	+ CCL5-Migrated	15.1	15.9	26.9	19.4	18.8	0.02

^*indicates that p-values were ascertained relative to the input MFI. Key: aIVBBB: cytokine-activated in vitro blood-brain barrier, IVBBB: in vitro blood-brain barrier, n/a: not applicable, n.s.: not significant.