Table 3.

Quantification of CCL5-driven T-cell migration across basal IVBBB and aIVBBB. This table compliments Figure 3, demonstrating significant reduction in the average CCR5 mean fluorescent intensity (MFI) on CD3+ T-cells following CCL5-driven migration across either the basal IVBBB or aIVBBB. The reduction in CCR5 MFI on migrated CD3+ T-cells was specific for CCL5, as there was no reduction seen in T-cells that migrated without added chemokine or in response to CCL2, a non-CCR5 binding chemokine. As demonstrated in Table 2a, endothelial activation significantly facilitated CCL5-driven CD3+ migration with selective migration of CCR5+ cells relative to the input in these series of experiments. The high percentages of CCR5+ T-cells that migrated in response to CCL2 most likely reflect a selective population of CCR2+ CCR5+ CD3+ T-cells. [ ] indicates the mean percentage of input T-cells that migrated in 3 hours, while ( ) indicates the mean percentage of CD3+ T-cells that were CCR5+.

BBB MODEL	CONDITION	CD3+	CD3+ CCR5+	MFI	p-value*
	INPUT	478,957	87,203 (18.21%)	43.9	n/a
Basal IVBBB	No Chemokine	3,419 [0.71%]	1,059 (31.0%)	43.8	n.s.
Basal IVBBB	+ CCL2	10,567 [2.21%]	7,429 (70.3%)	42.4	n.s.
Basal IVBBB	+ CCL5	18,328 [3.83%]	10,021 (54.7%)	26.3	0.02
aIVBBB	No Chemokine	5,713 [1.19%]	2,216 (38.8%)	42.0	n.s.
aIVBBB	+ CCL2	26,930 [5.62%]	19,746 (73.3%)	41.3	n.s.
aIVBBB	+ CCL5	33,588 [7.01%]	18,438 (54.9%)	26.8	0.03

^*indicates that p-values were ascertained relative to the mean input MFI (n=3). Key: aIVBBB: cytokine-activated in vitro blood-brain barrier, IVBBB: in vitro blood-brain barrier.