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Solid-state NMR spectra of samples used in this work. Spectra of Rnq1153–405 labeled with Tyr-1–13C, Leu-1–13C, or Ala-3–13C were recorded at 9.39 T at 20 kHz MAS. The carbonyl peak of the Leu-1–13C-labeled sample was asymmetric, and could be deconvoluted into two Gaussian peaks.