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. 2008 Feb 4;105(7):2421–2426. doi: 10.1073/pnas.0707082105

Fig. 3.

Fig. 3.

COUP-TFII is required for hedgehog-mediated suppression of adipogenesis. (A) The 3T3-L1 preadipocytes were treated with indicated amount of Shh for 48 h. Cell lysates were subjected to Western blotting for COUP-TFII and β-actin. (B) The 3T3-L1 preadipocytes retrovirally transduced with shRNA specific for COUP-TFII (shCOUP) or luciferase (shLuc) were induced with a mixture of 1.7 μM insulin, 10 μM dexamethasone, and 5 nM IBMX in the absence (−) or presence (+) of 300 ng/ml Shh. (C) The 3T3-L1 preadipocytes transduced with pMSCV empty vector or COUP-TFII were induced with a DMI mixture in the absence (−) or presence (+) of 3.6 μM KAAD-cyclopamine, an antagonist of hedgehog signaling. (B and C) Oil red O staining was performed 7 days after induction.