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. 2008 Feb 4;105(7):2421–2426. doi: 10.1073/pnas.0707082105

Fig. 4.

Fig. 4.

COUP-TFII is required for GATA-mediated suppression of adipogenesis. (A) The 3T3-L1 preadipocytes were transduced with GATA2-pMSCV or empty vector, selected, and then transduced a second time with a virus expressing shRNA specific for luciferase (shLuc) or COUP-TFII (shCOUP). Oil red O staining was performed 7 days after DMI induction. (B) HEK-293 cells were transfected with FLAG empty vector (Flag), FLAG-GATA2, or FLAG-GATA3. Co-IP analysis was performed with anti-FLAG beads. Ten percent input and the SDS eluate were subjected to Western blotting with polyclonal antibodies against FLAG or COUP-TFII. (C) The 293 cells were transfected with FLAG empty vector (EV) or FLAG-tagged deletion mutants of GATA2. Co-IP analysis was performed as described above. (D) The 3T3-L1 adipocytes (day 5 after DMI induction) were transfected with 1 μg of pCDNA3 (EV), 1 μg of COUP-TFII (COUP), 1 μg of GATA2 (GATA), or 0.5 μg of COUP-TFII plus 0.5 μg of GATA2 (COUP + GATA). Relative mRNA levels of C/EBPα and Glut4 were analyzed by using Q-PCR. Data are shown as mean ± SD of three biological replicates. *, P < 0.05; **, P < 0.01.