Fig. 4.

Analysis of gene expression in mouse liver tissues and human HCC tissues and cell lines. (A, C, and D) Immunohistochemical labeling demonstrates low/absent expression of phosphorylated Stat3 in normal (wild-type) mouse liver (A), ITIH4^−/− liver (C), and Elf^+/−/ITIH4^−/− liver (D). (B) In contrast, Elf^+/− HCC liver tissue shows increased expression of P-Stat3. (E and F) Immunohistochemical detection shows increased expression of Stat3 in human HCC tissues (F, arrows) compared with normal liver tissues (E). (H and G) Phosphorylated-Stat3 is also increased in human HCC tissues (H, arrows) compared with normal liver tissues (G). (Scale bar is in micrometers.)