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The kif4a promoter is much weaker than the cytomegalovirus (CMV) promoter. (a) Examples of the flow cytometry analysis. Medians for GFP-positive cells (transfected cells) are shown. (b) Comparative analysis of different promoters by using flow cytometry after transient transfection of tetO-GFP lamin A and various tTA vectors. Median values (as in a) are normalized to 1.0 for CMV-tTA2. Error bars represent SD (n ≥ 3).
